913 research outputs found
Biologically active Phytophthora mating hormone prepared by catalytic asymmetric total synthesis
A Phytophthora mating hormone with an array of 1,5-stereogenic centers has been synthesized by using our recently developed methodology of catalytic enantioselective conjugate addition of Grignard reagents. We applied this methodology in a diastereo- and enantioselective iterative route and obtained two of the 16 possible stereoisomers of Phytophthora hormone α1. These synthetic stereoisomers induced the formation of sexual spores (oospores) in A2 mating type strains of three heterothallic Phytophthora species, P. infestans, P. capsici, and P. nicotianae but not in A1 mating type strains. The response was concentration-dependent, and the oospores were viable. These results demonstrate that the biological activity of the synthetic hormone resembles that of the natural hormone α1. Mating hormones are essential components in the sexual life cycle of a variety of organisms. For plant pathogens like Phytophthora, sexual reproduction is important as a source of genetic variation. Moreover, the thick-walled oospores are the most durable propagules that can survive harsh environmental conditions. Sexual reproduction can thus greatly affect disease epidemics. The availability of synthetic compounds mimicking the activity of Phytophthora mating hormone will be instrumental for further unravelling sexual reproduction in this important group of plant pathogens.
Assessing the reduction of the hydrological connectivity of gully systems through vegetation restoration: field experiments and numerical modelling
Restoration of degraded land in the Southern Ecuadorian Andes has led to alterations in the functioning of degraded catchments. Recovery of vegetation on areas affected by overgrazing, as well as the reforestation or afforestation of gully areas have given rise to modifications of hydrological connectivity within the catchments. Recent research has highlighted the ability of gully channels to trap sediment eroded from steep slopes, especially if vegetation is established along the gully bed. However, vegetation cover not only induces sediment deposition in the gully bed, but may also have a potential to reduce runoff water volume. The performance of gully beds in reducing the transfer of runoff was investigated by conducting controlled concentrated flow experiments in the field. Experimental field data for nine gullies were derived by pouring concentrated inflow into the upstream end and measuring the outflow at the downstream end of the channel. Two consecutive flow experiments per gully were carried out, so that data for dry and wet soil conditions were collected. The hydrological response to concentrated flow was estimated for each experiment by calculating its cumulative infiltration coefficient, <i>IC</i> (%). The results showed a great difference in <i>IC</i> between dry and wet soil conditions. The <i>IC</i> for wet soil conditions was on average 24%, whereas it was 60% for dry conditions. Gullies with more than 50% surface vegetation cover exhibit the highest cumulative infiltration coefficients (81% for dry runs, and 34% for wet runs), but runoff transmission losses were not as clearly related to vegetation cover as sediment storage as shown in Molina et al. (2009). The experimental field data of 16 experiments were used to calibrate a hydrological model developed by Fiener and Auerswald (2005) in order to simulate the transfer of concentrated flow along the gully beds. The calibrated model was able to simulate the transfer of runoff water well, as the error on the simulated total outflow volumes is below 13% for 15 out of 16 cases. However, predicting infiltration amounts is difficult: the high sensitivity of model results to some crucial hydraulic parameters (runoff width, hydraulic conductivity and sorptivity) is one of the reasons why the relationships between model parameter values and gully features are relatively weak. <br><br> The results obtained from the field experiments show that gully systems are key elements in the hydrological connectivity of degraded landscapes. The transfer of overland flow and sediment from the slopes towards the river system highly depends on the presence/absence of vegetation in the gully beds and should therefore be accounted for in assessments of landscape degradation and/or recovery
TWINLATIN: Twinning European and Latin-American river basins for research enabling sustainable water resources management. Combined Report D3.1 Hydrological modelling report and D3.2 Evaluation report
Water use has almost tripled over the past 50 years and in some regions the water demand already
exceeds supply (Vorosmarty et al., 2000). The world is facing a “global water crisis”; in many
countries, current levels of water use are unsustainable, with systems vulnerable to collapse from even
small changes in water availability. The need for a scientifically-based assessment of the potential
impacts on water resources of future changes, as a basis for society to adapt to such changes, is strong
for most parts of the world. Although the focus of such assessments has tended to be climate change,
socio-economic changes can have as significant an impact on water availability across the four main
use sectors i.e. domestic, agricultural, industrial (including energy) and environmental. Withdrawal
and consumption of water is expected to continue to grow substantially over the next 20-50 years
(Cosgrove & Rijsberman, 2002), and consequent changes in availability may drastically affect society
and economies.
One of the most needed improvements in Latin American river basin management is a higher level of
detail in hydrological modelling and erosion risk assessment, as a basis for identification and analysis
of mitigation actions, as well as for analysis of global change scenarios. Flow measurements are too
costly to be realised at more than a few locations, which means that modelled data are required for the
rest of the basin. Hence, TWINLATIN Work Package 3 “Hydrological modelling and extremes” was
formulated to provide methods and tools to be used by other WPs, in particular WP6 on “Pollution
pressure and impact analysis” and WP8 on “Change effects and vulnerability assessment”. With an
emphasis on high and low flows and their impacts, WP3 was originally called “Hydrological
modelling, flooding, erosion, water scarcity and water abstraction”. However, at the TWINLATIN
kick-off meeting it was agreed that some of these issues resided more appropriately in WP6 and WP8,
and so WP3 was renamed to focus on hydrological modelling and hydrological extremes.
The specific objectives of WP3 as set out in the Description of Work are
Mitochondrial DNA mutations in renal disease: an overview
Kidneys have a high energy demand to facilitate the reabsorption of the glomerular filtrate. For this reason, renal cells have a high
density of mitochondria. Mitochondrial cytopathies can be the result of a mutation in both mitochondrial and nuclear DNA.
Mitochondrial dysfunction can lead to a variety of renal manifestations. Examples of tubular manifestations are renal Fanconi
Syndrome, which is often found in patients diagnosed with Kearns-Sayre and Pearson’s marrow-pancreas syndrome, and distal
tubulopathies, which result in electrolyte disturbances such as hypomagnesemia. Nephrotic syndrome can be a glomerular
manifestation of mitochondrial dysfunction and is typically associated with focal segmental glomerular sclerosis on histology.
Tubulointerstitial nephritis can also be seen in mitochondrial cytopathies and may lead to end-stage renal disease. The underlying
mechanisms of these cytopathies remain incompletely understood; therefore, current therapies focus mainly on symptom relief. A
better understanding of the molecular disease mechanisms is critical in order to improve treatments
Validation of Soil Erosion Risk Assessments in Italy.
Abstract not availableJRC.H-Institute for environment and sustainability (Ispra
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